Journal: iScience

Article Title: Ultra-large scale virtual screening identifies a small molecule inhibitor of the Wnt transporter Wntless

doi: 10.1016/j.isci.2024.110454

Figure Lengend Snippet:

Article Snippet: 40 μL of protein A/G plus agarose (#sc-2003, Santa Cruz) was added the next day and incubated for another 2 h before washing and elution.

Techniques: Recombinant, Protease Inhibitor, Western Blot, Luciferase, Bicinchoninic Acid Protein Assay, Knock-Out, Software

Journal: eLife

Article Title: Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice

doi: 10.7554/eLife.62174

Figure Lengend Snippet:

Article Snippet: Precleared homogenates were subjected to immunoprecipitation using 1 μg of the anti-NCOA4 antibody (Sigma-Aldrich, SAB1404569) or mouse immunoglobulin G (IgG; Santa Cruz biochemistry, sc-2025) and 40 μL of magnetic beads-coupled protein G at 4 °C for 2 hr.

Techniques: Western Blot, Immunofluorescence, Diagnostic Assay, Magnetic Beads, SYBR Green Assay, Staining, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Iron Assay, Multiple Displacement Amplification, Glutamate Assay, Electrophoretic Mobility Shift Assay, PCR Cloning, Software